RNA
Part:BBa_K2923001
Designed by: claire husser Group: iGEM19_Strasbourg (2019-09-12)
Theophylline actif aptazyme
An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme sequence is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. Aptazyme is composed of 3 different parts: a twister ribozyme module, a communication module and an aptamer module.
The described anti-theophylline aptazyme is an switch-off. Its catalytic activity (self-cleavage) requires the absence of ligand.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//classic/rna/uncategorized
ligands | Theophylline |
switch_point | 2.5 mM of Theophylline |